| 品牌 | 自营品牌 | 货号 | BFN60808733 |
|---|---|---|---|
| 规格 | T25培养瓶x1 1.5ml冻存管x2 | 供货周期 | 现货 |
| 主要用途 | 仅供科研 | 应用领域 | 医疗卫生,生物产业 |
细胞名称 | 人肝永生化细胞THLE-2 |
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货物编码 | BFN60808733 | ||
产品规格 | T25培养瓶x1 | 1.5ml冻存管x2 | |
细胞数量 | 1x10^6 | 1x10^6 | |
保存温度 | 37℃ | -198℃ | |
运输方式 | 常温保温运输 | 干冰运输 | |
安全等级 | 1 | ||
用途限制 | 仅供科研 2类 | ||
培养体系 | DMEM高糖+10%FBS+1%三抗 | ||
培养温度 | 37℃ | 二氧化碳浓度 | 5% |
简介 | 人肝永生化细胞THLE-2取自男性供体，经过SV40永生化处理。该细胞源于ATCC | ||
注释 | 倍增时间33h | ||
基因突变 | / | ||
HLA信息 |
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STR信息 | Amelogenin X,Y CSF1PO 11,13 D5S818 11,13 D7S820 10,12 D13S317 8,12 D16S539 11,13 TH01 7,9.3 TPOX 8,11 vWA 16,17 | ||
参考文献 | PubMed=7685115; DOI=10.1073/pnas.90.11.5123 Pfeifer A.M.A., Cole K.E., Smoot D.T., Weston A., Groopman J.D., Shields P.G., Vignaud J.M., Juillerat M., Lipsky M.M., Trump B.F., Lechner J.F., Harris C.C. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. U.S.A. 90:5123-5127(1993)
PubMed=8504475; DOI=10.1093/carcin/14.5.833 Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C. p53 mutations in human immortalized epithelial cell lines. Carcinogenesis 14:833-839(1993)
Patent=US5759765 Harris C.C., Cole K.H., Lechner J.F., Reddel R.R. Human liver epithelial cell lines. Patent number US5759765, 02-Jun-1998
PubMed=23887712; DOI=10.1038/ncomms3218 Nault J.-C., Mallet M., Pilati C., Calderaro J., Bioulac-Sage P., Laurent C., Laurent A., Cherqui D., Balabaud C., Zucman-Rossi J. High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions. Nat. Commun. 4:2218-2218(2013)
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