人黑色素瘤细胞WM35

细胞名称

人黑色素瘤细胞WM35

货物编码

BFN60808633

产品规格

T25培养瓶x1

1.5ml冻存管x2

细胞数量

1x10^6

1x10^6

保存温度

37℃

-198℃

运输方式

常温保温运输

干冰运输

安全等级

1

用途限制

仅供科研 2类

培养体系

DMEM高糖+10%FBS+1%三抗

培养温度

37℃

二氧化碳浓度

5%

简介

人黑色素瘤细胞WM35取自24岁女性供体

注释

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: Wistar Institute melanoma cell line collection.

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: DNA methylation analysis.

Omics: Transcriptome analysis.

Caution: The reported STR profile from Wistar of this cell line was changed at one point between February 2016 when we retrieved them and entered them in the Cellosaurus and May 2018. The major changes were: D18S51: 20->14,15 and FGA: 19->19,24.

基因突变

Heterozygous for BRAF p.Val600Glu (c.1799T>A) (PubMed=18632627; Wistar).

HLA信息

/

STR信息

Amelogenin X

CSF1PO 10

D2S1338 18,25

D3S1358 16,18

D5S818 11,12

D7S820 10,13

D8S1179 11,13

D13S317 11

D16S539 12,13

D18S51 14,15

D19S433 15,16

D21S11 29,32.2

FGA 19,24

TH01 9.3

TPOX 8

vWA 17

参考文献

PubMed=23039341; DOI=10.1186/1476-4598-11-75

Byron S.A., Loch D.C., Wellens C.L., Wortmann A., Wu J., Wang J., Nomoto K., Pollock P.M.

Sensitivity to the MEK inhibitor E6201 in melanoma cells is associated with mutant BRAF and wildtype PTEN status.

Mol. Cancer 11:75-75(2012)

PubMed=27087056; DOI=10.1038ep24569

Haridas P., McGovern J.A., Kashyap A.S., McElwain D.L.S., Simpson M.J.

Standard melanoma-associated markers do not identify the MM127 metastatic melanoma cell line.

Sci. Rep. 6:24569-24569(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)