| 品牌 | 自营品牌 | 货号 | BFN60806666 |
|---|---|---|---|
| 规格 | T25培养瓶x1 1.5ml冻存管x2 | 供货周期 | 现货 |
| 主要用途 | 仅供科研 | 应用领域 | 医疗卫生,生物产业 |
细胞名称 | 人食管上皮细胞HET-1A |
| |
货物编码 | BFN60806666 | ||
产品规格 | T25培养瓶x1 | 1.5ml冻存管x2 | |
细胞数量 | 1x10^6 | 1x10^6 | |
保存温度 | 37℃ | -198℃ | |
运输方式 | 常温保温运输 | 干冰运输 | |
安全等级 | 1 | ||
用途限制 | 仅供科研 1类 | ||
培养体系 | DMEM高糖+10%FBS+1%双抗 | ||
培养温度 | 37℃ | 二氧化碳浓度 | 5% |
简介 | 人食管上皮细胞HET-1A取自25岁男性。 | ||
序列突变 | / | ||
注释 | Doubling time: 27.4 +- 3.6 hours (PubMed=21127259). Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [pRSV-T]. Omics: Deep RNAseq analysis. Omics: Transcriptome analysis. | ||
STR信息 | Amelogenin X,Y CSF1PO 10,12 D5S818 11,12 D7S820 9 D13S317 11 D16S539 9,11 TH01 7 TPOX 11 vWA 16 | ||
参考文献 | Patent=WO1991005062A1 Stoner G.D., Reddel R.R., Harris C.C. Human esophageal epithelial cell lines. Patent number WO1991005062A1, 18-Apr-1991
PubMed=1703038 Stoner G.D., Kaighn M.E., Reddel R.R., Resau J.H., Bowman D., Naito Z., Matsukura N., You M., Galati A.J., Harris C.C. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51:365-371(1991)
PubMed=8504475; DOI=10.1093/carcin/14.5.833 Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C. p53 mutations in human immortalized epithelial cell lines. Carcinogenesis 14:833-839(1993)
PubMed=20139130; DOI=10.1093/carcin/bgq036 Duggan S.P., Behan F.M., Kirca M., Smith S.M., Reynolds J.V., Long A., Kelleher D.P. An integrative genomic approach in oesophageal cells identifies TRB3 as a bile acid responsive gene, downregulated in Barrett's oesophagus, which regulates NF-kappaB activation and cytokine levels. Carcinogenesis 31:936-945(2010)
PubMed=20843300; DOI=10.1042/BC20100071 Underwood T.J., Derouet M.F., White M.J., Noble F., Moutasim K.A., Smith E., Drew P.A., Thomas G.J., Primrose J.N., Blaydes J.P. A comparison of primary oesophageal squamous epithelial cells with HET-1A in organotypic culture. Biol. Cell 102:635-644(2010)
PubMed=21127259; DOI=10.1152/ajpgi.00461.2010 Goldman A., Chen H.D.R., Roesly H.B., Hill K.A., Tome M.E., Dvorak B., Bernstein H., Dvorak K. Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis. Am. J. Physiol. Gastrointest. Liver Physiol. 300:G292-G302(2011)
PubMed=28224081; DOI=10.1016/j.gdata.2017.02.002 Akagi I., Ishibashi O. RNA-Seq analysis of human cell lines established from normal and neoplastic esophageal squamous epithelium. Genomics Data 12:4-6(2017) | ||
青旗（上海）生物技术发展有限公司，总部位于上海浦东新区，依托本地高校资源，逐步发展成为以生物技术为主的研发、生产、培训为一体的综合化产业平台，在标准化细胞库建立及细胞药物前端模型方面成果显著。公司生产经营原代细胞、细胞系、ELISA试剂盒、感受态细胞和HPLC检测等科研产品与服务。我们秉承对用户负责的态度，以对科研的高度严谨，以严格的质量控制，为广大生物医学科研用户提供更优质的服务！